Purpose: To measure the prevalence and degree of burnout among nursing staff working in Beni-Suef University Hospital. Methods: A cross-sectional study was conducted from June–December 2016 on a group of nurses (n = 286) with a response rate of 79.4% (227/286). The Maslach Burnout Inventory-Human Service Survey tool was used to estimate the prevalence and degree of burnout. Results: The mean age in the current study was 28.9 ± 7.8 years and the mean number of years of experience was 9.5 ± 7.7. Of the nurses, 43.2% were classified as having a moderate level of burnout and 32.6% as having a high level of burnout; 54.6% of nurses had average levels of emotional exhaustion, 48% scored high on depersonalization, and 77.5% had high levels of reduced personal accomplishment. The number of shifts, type of violence, shift timing, and quality of life perception proved to be significant predictors for high grades of burnout among nurses. Conclusion: Burnout appeared to be common among participating nurses. Searching for and eliminating sources of stress in hospitals, in addition to teaching nurses coping strategies to deal with stress at work, seem to be important steps. (PsycINFO Database Record (c) 2017 APA, all rights reserved